Reprotox supports the 3Rs (Replacement, Reduction, Refinement) by advancing scientifically robust in vitro alternatives.

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Mon - Fri: 9:00 am - 5:00 pm
800 Bradbury Dr. SE, Science & Technology Park, Albuquerque, NM 87106
  • [email protected]
  • 800 Bradbury Dr. SE, Albuquerque, NM 87106
  • Mon - Fri: 9:00 am - 5:00 pm
Call +1 (505) 333-9178
3D In Vitro Reproductive Toxicity Testing

Advanced In Vitro Reproductive Toxicology Services

Predictive 3D Mini-Testis Models for Mechanistic Screening

Moving beyond traditional 2D assays, Reprotox Biotech provides a physiologically relevant 3D mini-testis model that recapitulates the complex testicular niche. Our multi-cellular co-culture system includes Spermatogonia, Sertoli, and Leydig cells embedded in a specialized extracellular matrix (ECM) to mimic in vivo morphology and paracrine signaling. These animal-free reproductive toxicity services are aligned with New Approach Methodologies (NAMs) and support mechanism-informed screening in male reproductive toxicology.

  • High-Content Analysis (HCA): Multi-parametric phenotypic profiling of 10+ endpoints, including nuclear morphology, cytoskeletal integrity (F-actin), and DNA damage response (γH2AX).
  • Regulatory Alignment: Validated as a New Approach Methodology (NAM), supporting the 3Rs principles by reducing reliance on animal models.
  • Exceptional Accuracy: Validated models demonstrate 91.5% sensitivity and 93.8% specificity compared to traditional in vivo reproductive toxicity data.
Request a Custom Study Design View Assays

Animal-free murine 3D-M®Repro

ReproTox has developed an animal-free 3D testicular cell co-culture system (3D-T®Repro) from a combination of testicular cell lines. This model has gone through extensive biomarker and morphology testing to ensure that it reflects in vitro conditions and provides affordable, humane, and efficient methods for reproductive and developmental toxicity screening.

3D-T®Repro_Primary

ReproTox has developed a proprietary technology to establish in vitro 3D testicular models at different stages of spermatogenesis from mice, rats, and canine. We derive testicular cells from neonate, juvenile, and adolescent sources to establish testicular-like organoids representing differential stages of spermatogenesis.

This organoid culture recapitulates many in vivo features and enables assessment of differentiation and mitosis. An extensive panel of toxicological endpoints is assessed in multiple cell types and at multiple time points using High-Content Screening (HCS). By combining these endpoints with exposure levels, the system delivers a sensitive and specific prediction of repro-toxicity.

3D-T®Repro_EX-Vivo

3D-T®Repro_EX-Vivo provides an innovative technology to culture seminiferous tubules and enable spermatogenesis in vitro. The technology preserves the original structure of the seminiferous tubules, and Sertoli cells remain polarized—crucial for germ cell differentiation—allowing the study of both mitotic and meiotic phases.

High-Content BioAssay System

ReproTox is at the forefront of cell-based High-Throughput (HT) and pathway-based High-Content Analysis (HCA) for reproductive toxicity evaluations. We provide fully automated HT screening and high-content analysis, plus access to our reproductive toxicity database (150 compounds). Fluorescence-based microplate assays enable rapid, quantitative readouts suitable for High-Content Analysis.

High-Content Analysis (HCA)

Examples of In Vitro Assays

Assay No Name/Species Endpoint Cell Type Price
CS-SCs Canine Sertoli Cell Viability/Cytotoxicity, Multiplexed High-Content analysis Canine, Primary Sertoli Cell Quote
MU-SCs Mouse Sertoli Cell Viability/Cytotoxicity, Multiplexed High-Content analysis Mouse, Sertoli Cell Line Quote
MU-LCs Mouse Leydig Cell Viability/Cytotoxicity, Multiplexed High-Content analysis Mouse, Leydig Cell Line Quote
MU-SPs Mouse Spermatogonial Cell Viability/Cytotoxicity, Multiplexed High-Content analysis Mouse, Spermatogonial Cell Quote
HU-SCs Human Sertoli Cell Viability/Cytotoxicity, Multiplexed High-Content analysis Human Primary Sertoli Cells Quote
HU-LCs Human Leydig Cell Viability/Cytotoxicity, Multiplexed High-Content analysis Human Primary Leydig Cells Quote
3D-T®Repro_Primary Canine Canine Organoid Viability/Cytotoxicity, Multiplexed High-Content analysis Canine Primary Testicular Cells Quote
3D-T®Repro_Primary Mouse Mouse reproductive 3D model Viability/Cytotoxicity, Multiplexed High-Content analysis Mouse primary testicular cells Quote
3D-T®Repro_Primary Rat Rat reproductive primary cell 3D model Multiplexed High-Content analysis Rat primary testicular cells Quote